SynMyco transposon: engineering transposon vectors for efficient transformation of minimal genomes
نویسندگان
چکیده
منابع مشابه
Transposon Occupation in Eukaryotic Genomes
Transposable elements, or transposons, are selfish genetic elements that possess the ability to mobilize within a genome, potentially causing serious damage. They can accomplish this through several well-understood mechanisms. Most move via an RNA intermediate (class I: retrotransposons), whereas others colonize new locations through the direct transfer of transposon DNA (class II: DNA transpos...
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Two new mariner transposon delivery vector systems, pMarA333 and pMarB333, had been constructed to randomly mutagenize Bacillus thuringiensis in vivo. The results showed that both systems could randomly insert into the genome of B. thuringiensis YBT881 (CCAM 020673). These systems could have potential for further construction of mutant libraries of B. thuringiensis and other Bacillus strains.
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Despite the presence of over 3 million transposons separated on average by approximately 500 bp, the human and mouse genomes each contain almost 1000 transposon-free regions (TFRs) over 10 kb in length. The majority of human TFRs correlate with orthologous TFRs in the mouse, despite the fact that most transposons are lineage specific. Many human TFRs also overlap with orthologous TFRs in the ma...
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Gene therapy offers important perspectives in current and future medicine but suffers from imperfect vectors for the delivery of the therapeutic gene. Most preclinical and clinical trials have been based on the use of viral vectors, which have evident advantages but also some serious disadvantages. In the past decade the use of DNA transposon-based systems for gene delivery has emerged as a non...
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Transposable elements are natural, non-viral gene delivery vehicles capable of mediating stable genomic integration. The Sleeping Beauty (SB) transposon has the ability to cut-and-paste the ‘gene of interest’ into the genome, providing the basis for long-term, permanent transgene expression in transgenic cells and organisms. The SB transposon system is relatively well characterized, and has bee...
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ژورنال
عنوان ژورنال: DNA Research
سال: 2019
ISSN: 1340-2838,1756-1663
DOI: 10.1093/dnares/dsz012